Hotspot

trnR-ACG-trnN-GUU

IRb · 110741-111364 · 624 bp

Back to Hesperaloe

Pi 0.00481

MarkerSeek score 61.50

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 624
variable_sites: 3
pi: 0.00481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	765 bp	1.0	78.6
trnR-ACG-trnN-GUU_p2	GAACAGATCCTGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	802 bp	1.0	78.0
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	716 bp	1.0	77.4
trnR-ACG-trnN-GUU_p4	GGGAACAGATCCTGTGGAGA	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.4	58.0	804 bp	1.0	76.9
trnR-ACG-trnN-GUU_p5	GAACAGATCCTGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	753 bp	1.0	76.8