Hotspot

petA-psbJ

LSC · 64881-65992 · 1112 bp

Back to Hesperaloe

Pi 0.00723

MarkerSeek score 63.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 8
pi: 0.00723
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.8	58.3	1243-1247 bp	1.0	77.1
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	57.8	57.6	1239-1243 bp	1.0	76.2
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	57.8	57.6	1242-1246 bp	1.0	76.2
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	CGGTTCATATTCCGGGTTGG	50.0	55.0	57.8	58.7	1244-1248 bp	1.0	74.0
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	TATTCCGGGTTGGGTTCATC	50.0	50.0	57.8	57.3	1237-1241 bp	1.0	73.8