Hotspot

ndhJ-ndhK

LSC · 50491-50565 · 75 bp

Back to Hesperaloe

Pi 0.0133

MarkerSeek score 60.20

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 75
variable_sites: 1
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	GCAATCGAAGCCCAAAGATC	CAGGAAAGGTTCTTTTGTGCA	50.0	42.9	57.5	57.2	171 bp	1.0	62.2
ndhJ-ndhK_p2	GAGTCCCAATCCTCGGTTTT	CAGGAAAGGTTCTTTTGTGCA	50.0	42.9	57.5	57.2	215 bp	1.0	62.0
ndhJ-ndhK_p3	GCAATCGAAGCCCAAAGATC	GGAAAGGTTCTTTTGTGCAGA	50.0	42.9	57.5	56.9	169 bp	1.0	60.0
ndhJ-ndhK_p4	GCAATCGAAGCCCAAAGATC	TCAGGAAAGGTTCTTTTGTGC	50.0	42.9	57.5	56.9	172 bp	1.0	60.0
ndhJ-ndhK_p5	GCAATCGAAGCCCAAAGATC	AGGAAAGGTTCTTTTGTGCAG	50.0	42.9	57.5	56.9	170 bp	1.0	59.9