Hotspot

psbM-trnD-GUC

LSC · 30606-31673 · 1068 bp

Back to Hesperaloe

Pi 0.00468

MarkerSeek score 61.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1068
variable_sites: 5
pi: 0.00468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	45.0	55.0	55.8	58.1	1196-1197 bp	1.0	62.0
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	45.0	50.0	55.8	58.1	1200-1201 bp	1.0	61.3
psbM-trnD-GUC_p3	AGTGCGAGAATATTGACTTCCA	CCTCGCTGGGATTGTAGTTC	40.9	55.0	57.5	58.1	1172-1173 bp	1.0	60.1
psbM-trnD-GUC_p4	AGTGCGAGAATATTGACTTCCA	TACACCTCGCTGGGATTGTA	40.9	50.0	57.5	58.1	1176-1177 bp	1.0	59.4
psbM-trnD-GUC_p5	AGGAACTAGAATGAACAGTGCA	CCTCGCTGGGATTGTAGTTC	40.9	55.0	57.4	58.1	1203-1204 bp	1.0	58.9