Hotspot

rpoB-trnC-GCA

LSC · 27152-28325 · 1174 bp

Back to Hesperaloe

Pi 0.0043

MarkerSeek score 62.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1174
variable_sites: 5
pi: 0.0043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TGAAATTCCTCCGACAAGCC	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1322-1325 bp	1.0	75.3
rpoB-trnC-GCA_p2	TGAAATTCCTCCGACAAGCC	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1359-1362 bp	1.0	73.3
rpoB-trnC-GCA_p3	TGAAATTCCTCCGACAAGCC	GACACCCGGATTTGAACTGG	50.0	55.0	58.2	58.8	1345-1348 bp	1.0	72.8
rpoB-trnC-GCA_p4	TGAAATTCCTCCGACAAGCC	CCGGATTTGAACTGGGGATA	50.0	50.0	58.2	57.0	1340-1343 bp	1.0	71.3
rpoB-trnC-GCA_p5	TGAAATTCCTCCGACAAGCC	ATAAAGGATTTGCAGTCCCCC	50.0	47.6	58.2	58.0	1323-1326 bp	1.0	71.2