Hotspot

rps16-trnQ-UUG

LSC · 5854-7299 · 1446 bp

Back to Hesperaloe

Pi 0.00347
MarkerSeek score 49.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1446
variable_sites
5
pi
0.00347
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1518-1519 bp 1.0 78.7
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GTCACTCGGAGGTTCGAATC 45.0 55.0 58.1 58.1 1526-1527 bp 1.0 78.6
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1529-1530 bp 1.0 78.2
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GTCACTCGGAGGTTCGAATC 50.0 55.0 58.2 58.1 1537-1538 bp 1.0 78.1
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1522-1523 bp 1.0 77.6