Hotspot

ndhH-rps15

SSC · 124827-124929 · 103 bp

Back to Herpetospermum

Pi 0.068

MarkerSeek score 69.70

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 103
variable_sites: 7
pi: 0.068
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	ATGAGTCGAAGAACACCGTG	CGGAAAACGCCAACGATTAC	50.0	50.0	57.9	58.1	292 bp	1.0	80.9
ndhH-rps15_p2	ATGAGTCGAAGAACACCGTG	TCGGAAAACGCCAACGATTA	50.0	45.0	57.9	58.2	293 bp	1.0	80.3
ndhH-rps15_p3	GGCCTGATAACCCCCAATTT	CGGAAAACGCCAACGATTAC	50.0	50.0	57.8	58.1	870 bp	1.0	79.2
ndhH-rps15_p4	ACTGTTCGGGACCATTTACG	CGGAAAACGCCAACGATTAC	50.0	50.0	57.9	58.1	471 bp	0.5	58.3
ndhH-rps15_p5	ACTGTTCGGGACCATTTACG	TCGGAAAACGCCAACGATTA	50.0	45.0	57.9	58.2	472 bp	0.5	57.6