Hotspot

ndhF-rpl32

SSC · 113969-114581 · 613 bp

Back to Herpetospermum

Pi 0.0351

MarkerSeek score 66.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 613
variable_sites: 21
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGGCGCACAAAGTAACAAAT	GGCGATGGGGATTCGTATTT	45.0	50.0	58.0	58.1	1750-1768 bp	1.0	82.1
ndhF-rpl32_p2	GTCGAAGGTTTTTGGTTGCC	GGCGATGGGGATTCGTATTT	50.0	50.0	58.2	58.1	1171-1189 bp	1.0	82.1
ndhF-rpl32_p3	CGCGATTAGTGACAAATGCC	GGCGATGGGGATTCGTATTT	50.0	50.0	57.9	58.1	1609-1627 bp	1.0	82.0
ndhF-rpl32_p4	TAGAACGGCGCACAAAGTAA	GGCGATGGGGATTCGTATTT	45.0	50.0	57.8	58.1	1755-1773 bp	1.0	81.2
ndhF-rpl32_p5	GAAGGTTTTTGGTTGCCGTC	GGCGATGGGGATTCGTATTT	50.0	50.0	58.2	58.1	1168 bp	0.5	57.9