Hotspot

ndhC-trnV-UAC

LSC · 51980-52706 · 727 bp

Back to Herpetospermum

Pi 0.0472
MarkerSeek score 77.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
727
variable_sites
34
pi
0.0472
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAACACTTGA GGTTCGAATCCGTATAGCCC 33.3 55.0 56.8 57.9 814-821 bp 1.0 44.0
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAACACTTGA CGGTTCGAATCCGTATAGCC 33.3 55.0 56.8 58.3 815-822 bp 1.0 44.0
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAACACTTG GGTTCGAATCCGTATAGCCC 34.8 55.0 55.5 57.9 814-821 bp 1.0 44.0
ndhC-trnV-UAC_p4 ATGCCAAAATAGGAATAACACTTGA GGTTCGAATCCGTATAGCCC 32.0 55.0 57.3 57.9 815-822 bp 1.0 44.0
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAACACTTGAT GGTTCGAATCCGTATAGCCC 32.0 55.0 57.3 57.9 814-821 bp 1.0 44.0