Hotspot

psbZ-trnG-GCC

LSC · 37020-37582 · 563 bp

Back to Herpetospermum

Pi 0.0538

MarkerSeek score 78.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 563
variable_sites: 29
pi: 0.0538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	GATGGTTGGTCGGGTAACAA	TACCATTCAACTATATCCGC	50.0	40.0	57.8	52.3	682-740 bp	1.0	46.0
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	TACCATTCAACTATATCCGC	55.0	40.0	58.0	52.3	690-748 bp	1.0	45.9
psbZ-trnG-GCC_p3	CTTCTCCTGATGGTTGGTCG	TTACCATTCAACTATATCCGC	55.0	38.1	58.0	53.2	691-749 bp	1.0	45.9
psbZ-trnG-GCC_p4	TTGCTTCTCCTGATGGTTGG	TACCATTCAACTATATCCGC	50.0	40.0	57.8	52.3	693-751 bp	1.0	45.9
psbZ-trnG-GCC_p5	CTTCTCCTGATGGTTGGTCG	TTTACCATTCAACTATATCCGC	55.0	36.4	58.0	54.1	692-750 bp	1.0	45.9