Hotspot

trnC-GCA-petN

LSC · 28101-28997 · 897 bp

Back to Herpetospermum

Pi 0.0236

MarkerSeek score 72.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 21
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TCTAGAGTCCACTTCGTCCC	50.0	55.0	58.1	57.9	1018-1029 bp	1.0	80.6
trnC-GCA-petN_p2	TGCAAATCCCTTTTTCCCCA	TCTAGAGTCCACTTCGTCCC	45.0	55.0	57.9	57.9	1030-1041 bp	1.0	80.4
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CGTCCCCATACTACGAGTGA	50.0	55.0	58.1	58.3	1004-1015 bp	1.0	79.0
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCGTCCCCATACTACGAGTG	50.0	55.0	58.1	58.3	1005-1016 bp	1.0	79.0
trnC-GCA-petN_p5	TGCAAATCCCTTTTTCCCCA	CGTCCCCATACTACGAGTGA	45.0	55.0	57.9	58.3	1016-1027 bp	1.0	78.8