Hotspot

psbK-psbI

LSC · 7138-7536 · 399 bp

Back to Herpetospermum

Pi 0.0375

MarkerSeek score 70.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 14
pi: 0.0375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GCTGCCGTAAGCTTTCGATA	TTACGTCCCGGGTCATTAGA	50.0	50.0	58.4	57.9	491-512 bp	1.0	81.6
psbK-psbI_p2	GCTGCCGTAAGCTTTCGATA	ACGTCCCGGGTCATTAGATA	50.0	50.0	58.4	57.6	489-510 bp	1.0	79.8
psbK-psbI_p3	GCTGCCGTAAGCTTTCGATA	TACGTCCCGGGTCATTAGAT	50.0	50.0	58.4	57.6	490-511 bp	1.0	79.8
psbK-psbI_p4	GCTGCCGTAAGCTTTCGATA	TTCTTCACGTCCCGGATTAC	50.0	50.0	58.4	57.3	507-528 bp	1.0	77.4
psbK-psbI_p5	GCTGCCGTAAGCTTTCGATA	CGTCCCGGGTCATTAGATAG	50.0	55.0	58.4	57.0	488-509 bp	1.0	74.7