Hotspot

rps16-trnQ-UUG

LSC · 5913-6512 · 600 bp

Back to Herpetospermum

Pi 0.0918

MarkerSeek score 88.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 600
variable_sites: 55
pi: 0.0918
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 88.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	673-893 bp	1.0	84.0
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	684-904 bp	1.0	83.4
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GACGACCAACCACTACCAAT	45.0	50.0	58.1	57.5	769-989 bp	1.0	80.2
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GACGACCAACCACTACCAAT	50.0	50.0	58.2	57.5	780-1000 bp	1.0	79.7
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	677-897 bp	1.0	79.2