Hotspot

ycf1

IRb · 114521-120172 · 5652 bp

Back to Heritiera

Pi 0.00298
MarkerSeek score 46.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
5652
variable_sites
33
pi
0.00298
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGGAATCTTTTGATCTGGGCA TTGTCTGTTCAAATTCGTGA 42.9 35.0 57.3 53.4 246 bp 0.75 30.3
ycf1_p2 AGGAATCTTTTGATCTGGGCA TGTCTGTTCAAATTCGTGAT 42.9 35.0 57.3 53.2 245 bp 0.75 30.3
ycf1_p3 AAGGAATCTTTTGATCTGGGCA TTGTCTGTTCAAATTCGTGA 40.9 35.0 57.9 53.4 247 bp 0.75 30.3
ycf1_p4 AGGAATCTTTTGATCTGGGCA ATTGTCTGTTCAAATTCGTGA 42.9 33.3 57.3 54.1 247 bp 0.75 30.3
ycf1_p5 AGGAATCTTTTGATCTGGGCA TTGTCTGTTCAAATTCGTGAT 42.9 33.3 57.3 54.1 246 bp 0.75 30.3