Hotspot

ndhD

SSC · 129478-130881 · 1404 bp

Back to Heritiera

Pi 0.00427

MarkerSeek score 49.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1404
variable_sites: 12
pi: 0.00427
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGACCGTATTCGCGATTT	AAACAACTCGAAGCATGGGT	45.0	45.0	58.0	58.0	2121-2129 bp	1.0	81.4
ndhD_p2	GTAAATTCCGCACTTGTGGC	AAACAACTCGAAGCATGGGT	50.0	45.0	58.0	58.0	2066-2074 bp	1.0	81.4
ndhD_p3	TTGGACCGTATTCGCGATTT	GGCATGAAACAACTCGAAGC	45.0	50.0	58.0	58.0	2127-2135 bp	1.0	81.3
ndhD_p4	TTGGACCGTATTCGCGATTT	AGAACGGGTTTTTCTGGTCC	45.0	50.0	58.0	58.0	2006-2007 bp	0.75	70.6
ndhD_p5	TTGGACCGTATTCGCGATTT	AGATGTGAATCCGCTTGTCC	45.0	50.0	58.0	58.0	2185-2187 bp	0.75	70.5