Hotspot

ccsA-ndhD

SSC · 129199-129477 · 279 bp

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Pi 0.0394

MarkerSeek score 67.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 279
variable_sites: 19
pi: 0.0394
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GTAAATTCCGCACTTGTGGC	TTTTTGATTCTGGGCCACGA	50.0	45.0	58.0	58.0	545-552 bp	1.0	84.1
ccsA-ndhD_p2	TTGGACCGTATTCGCGATTT	TTTTTGATTCTGGGCCACGA	45.0	45.0	58.0	58.0	600-607 bp	1.0	83.9
ccsA-ndhD_p3	TAAATTCCGCACTTGTGGCT	TTTTTGATTCTGGGCCACGA	45.0	45.0	58.1	58.0	544-551 bp	1.0	83.5
ccsA-ndhD_p4	TTACTTGGACCGTATTCGCG	TTTTTGATTCTGGGCCACGA	50.0	45.0	58.1	58.0	604-611 bp	1.0	83.3
ccsA-ndhD_p5	TGGCGCAATCCAATACATGA	TTTTTGATTCTGGGCCACGA	45.0	45.0	58.0	58.0	837-844 bp	1.0	82.7