Hotspot

rpl32-trnL-UAG

SSC · 126959-128047 · 1089 bp

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Pi 0.0124

MarkerSeek score 55.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1089
variable_sites: 26
pi: 0.0124
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGGCGTTGAAAGCTTTTTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1220-1238 bp	1.0	81.5
rpl32-trnL-UAG_p2	CGGCGTTGAAAGCTTTTTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	1221-1239 bp	1.0	81.5
rpl32-trnL-UAG_p3	CGGCGTTGAAAGCTTTTTCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	1230-1248 bp	1.0	80.5
rpl32-trnL-UAG_p4	CGGCGTTGAAAGCTTTTTCA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.2	57.8	1229-1247 bp	1.0	80.0
rpl32-trnL-UAG_p5	CGGCGTTGAAAGCTTTTTCA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.2	57.8	1228-1246 bp	1.0	80.0