Hotspot

psaI-ycf4

LSC · 63560-63969 · 410 bp

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Pi 0.0126

MarkerSeek score 60.10

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 10
pi: 0.0126
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.0	57.9	551-555 bp	1.0	80.4
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.0	57.9	554-558 bp	1.0	80.3
psaI-ycf4_p3	TAGGCCTAGTATTTCCGGCA	GGATAAAGGCCCAGCAGAAA	50.0	50.0	57.9	57.9	561-565 bp	1.0	79.8
psaI-ycf4_p4	TAGGCCTAGTATTTCCGGCA	AAAGGATAAAGGCCCAGCAG	50.0	50.0	57.9	57.9	564-568 bp	1.0	79.8
psaI-ycf4_p5	ACTTTCAGCAGCTTTCCCTC	GGATAAAGGCCCAGCAGAAA	50.0	50.0	58.1	57.9	598-602 bp	1.0	79.7