Hotspot

atpF-atpH

LSC · 13365-13992 · 628 bp

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Pi 0.00637

MarkerSeek score 64.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 628
variable_sites: 8
pi: 0.00637
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGTTGTAGCATTGGCACTT	50.0	45.0	57.9	58.0	736-742 bp	1.0	80.5
atpF-atpH_p2	CCAGTAACCCAAGGAAACGA	TGGTTGTAGCATTGGCACTT	50.0	45.0	57.4	58.0	709-715 bp	1.0	77.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	CTGGTTGTAGCATTGGCACT	50.0	50.0	57.9	58.5	737-743 bp	1.0	76.9
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	ATGGGCTGGTTGTAGCATTG	50.0	50.0	57.9	58.5	742-748 bp	1.0	76.4
atpF-atpH_p5	GGCCAGTAACCCAAGGAAAC	TGGTTGTAGCATTGGCACTT	55.0	45.0	58.7	58.0	711-717 bp	1.0	75.6