Hotspot

trnK-UUU-rps16

LSC · 4291-5187 · 897 bp

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Pi 0.00768
MarkerSeek score 67.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
897
variable_sites
13
pi
0.00768
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 1006-1025 bp 1.0 81.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 1005-1024 bp 1.0 81.9
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC AAAAGGAGCTCAACCCACAG 50.0 50.0 57.5 58.0 992-1011 bp 1.0 79.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAGGAAGGCGGGTGTTTTTA 50.0 45.0 58.2 57.6 966-985 bp 1.0 79.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TAAGGAAGGCGGGTGTTTTT 50.0 45.0 58.2 57.6 967-986 bp 1.0 79.1