Hotspot

ndhF

Genome · 112834-115071 · 2238 bp

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Pi 0.00792

MarkerSeek score 43.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2238
variable_sites: 47
pi: 0.00792
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTGAGGCTCTAGATACGGGA	GGGGGTAGGATCCGGTAAAT	50.0	55.0	57.0	58.3	2883-2940 bp	1.0	73.6
ndhF_p2	AAATTGGAAGGGCCTCTCTT	GGGGGTAGGATCCGGTAAAT	45.0	55.0	56.7	58.3	2541-2609 bp	1.0	71.9
ndhF_p3	AATTGGAAGGGCCTCTCTTT	GGGGGTAGGATCCGGTAAAT	45.0	55.0	56.7	58.3	2540-2608 bp	1.0	71.9
ndhF_p4	TGAGGCTCTAGATACGGGAT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	56.7	58.3	2882-2939 bp	1.0	71.7
ndhF_p5	CGAGTTTAAATTGGAAGGGCC	GGGGGTAGGATCCGGTAAAT	47.6	55.0	57.5	58.3	2548-2616 bp	1.0	70.3