Hotspot

ccsA-ndhD

Genome · 118273-118501 · 229 bp

Back to Heracleum

Pi 0.0486

MarkerSeek score 70.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 229
variable_sites: 22
pi: 0.0486
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGGCTACATAGTTATGGTTCA	CAGTTGACAAGGTCGAGGTT	42.9	50.0	54.8	57.8	312-336 bp	1.0	51.7
ccsA-ndhD_p2	GGGCTACATAGTTATGGTTCA	AGTTGACAAGGTCGAGGTTC	42.9	50.0	54.8	57.5	311-335 bp	1.0	49.4
ccsA-ndhD_p3	GGGCTACATAGTTATGGTTCA	GTTGACAAGGTCGAGGTTCT	42.9	50.0	54.8	57.5	310-334 bp	1.0	49.4
ccsA-ndhD_p4	GGGCTACATAGTTATGGTTCA	TCAGTTGACAAGGTCGAGGT	42.9	50.0	54.8	58.6	313-337 bp	1.0	48.9
ccsA-ndhD_p5	GGGCTACATAGTTATGGTTCAT	CAGTTGACAAGGTCGAGGTT	40.9	50.0	55.4	57.8	312-336 bp	1.0	48.3