Hotspot

trnL-UAG-ccsA

Genome · 117202-117297 · 96 bp

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Pi 0.037

MarkerSeek score 66.00

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: Genome
length_bp: 96
variable_sites: 10
pi: 0.037
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	ACAATGGAAACGATCGAAAAGG	50.0	40.9	58.0	57.6	227-229 bp	1.0	64.8
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	ACAATGGAAACGATCGAAAAGG	50.0	40.9	58.0	57.6	226-228 bp	1.0	64.8
trnL-UAG-ccsA_p3	CTTAGGAAGCAGTGCGAGAG	ACAATGGAAACGATCGAAAAGG	55.0	40.9	58.1	57.6	204-206 bp	1.0	64.7
trnL-UAG-ccsA_p4	CTCTTAGGAAGCAGTGCGAG	ACAATGGAAACGATCGAAAAGG	55.0	40.9	58.1	57.6	206-208 bp	1.0	64.6
trnL-UAG-ccsA_p5	TGAATTCGAACAAGCCGCTA	ACAATGGAAACGATCGAAAAGG	45.0	40.9	57.9	57.6	249-251 bp	1.0	63.8