Hotspot

psbB-psbT

Genome · 74703-74913 · 211 bp

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Pi 0.035

MarkerSeek score 59.50

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: Genome
length_bp: 211
variable_sites: 16
pi: 0.035
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGTGCTAGAACCCTGTTCAG	ACCTAGAGTCGAGACTAAGAGG	55.0	50.0	57.9	57.6	365-377 bp	1.0	65.7
psbB-psbT_p2	GGTGCTAGAACCCTGTTCAG	CCTAGAGTCGAGACTAAGAGGA	55.0	50.0	57.9	57.3	364-376 bp	1.0	63.6
psbB-psbT_p3	GGTGCTAGAACCCTGTTCAG	CCTAGAGTCGAGACTAAGAGGAA	55.0	47.8	57.9	58.0	364-376 bp	1.0	60.8
psbB-psbT_p4	GGTGCTAGAACCCTGTTCAG	AGAGTCGAGACTAAGAGGAATGT	55.0	43.5	57.9	58.1	361-373 bp	1.0	59.9
psbB-psbT_p5	GGTGCTAGAACCCTGTTCAG	CCTAGAGTCGAGACTAAGAGG	55.0	52.4	57.9	55.8	364-376 bp	1.0	59.8