Hotspot

trnE-UUC-trnT-GGU

Genome · 31242-32043 · 802 bp

Back to Heracleum

Pi 0.028
MarkerSeek score 69.20
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
Genome
length_bp
802
variable_sites
47
pi
0.028
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.96
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GGCGAAACGAAAAGGGAAAG GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1359-1395 bp 1.0 85.9
trnE-UUC-trnT-GGU_p2 GAATGGCGAAACGAAAAGGG GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1363-1399 bp 0.888889 81.4
trnE-UUC-trnT-GGU_p3 GAATGGCGAAACGAAAAGGG TGGTGATTCCAGAGACGACT 50.0 50.0 58.0 58.1 1607-1671 bp 0.888889 81.4
trnE-UUC-trnT-GGU_p4 GAATGGCGAAACGAAAAGGG GGCGTTACTCTACCGTTGAG 50.0 55.0 58.0 58.1 1338-1374 bp 0.444444 61.0
trnE-UUC-trnT-GGU_p5 CCTGTTCCCTTGTGTTGACA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 1341 bp 0.111111 44.0