Hotspot

petN-psbM

Genome · 28850-30006 · 1157 bp

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Pi 0.0134

MarkerSeek score 62.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 1157
variable_sites: 38
pi: 0.0134
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	TGTAAAAACAGAAAGTCAAAATCA	50.0	25.0	57.6	53.9	1131-1223 bp	1.0	46.5
petN-psbM_p2	TGGGGAAGAAGTGGACTCTA	TGTAAAAACAGAAAGTCAAAATCA	50.0	25.0	57.0	53.9	1130-1222 bp	1.0	46.5
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	GTAAAAACAGAAAGTCAAAATCA	50.0	26.1	57.6	52.1	1130-1222 bp	1.0	46.5
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGTAAAAACAGAAAGTCAAAATC	50.0	26.1	57.6	52.1	1131-1223 bp	1.0	46.5
petN-psbM_p5	TGGGGAAGAAGTGGACTCTAG	TGTAAAAACAGAAAGTCAAAATCA	52.4	25.0	58.2	53.9	1130-1222 bp	1.0	46.5