Hotspot

rps16-trnQ-UUG

Genome · 5742-6996 · 1255 bp

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Pi 0.0181
MarkerSeek score 64.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
Genome
length_bp
1255
variable_sites
57
pi
0.0181
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1334-1394 bp 1.0 84.6
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1335-1395 bp 1.0 84.6
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AAATATAGATGGGGCGTGGC 45.0 50.0 58.1 57.8 1345-1405 bp 1.0 83.8
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 1360-1420 bp 1.0 81.9
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 1359-1419 bp 1.0 81.9