Hotspot

ycf1

SSC · 126546-132179 · 5634 bp

Back to Heptapleurum

Pi 0.0142

MarkerSeek score 45.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5634
variable_sites: 297
pi: 0.0142
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCGCGGCTTTTCCTTTAT	AGGATGTCCTTGCCCATACT	45.0	50.0	57.9	58.1	998-1091 bp	0.5	66.6
ycf1_p2	GTTTGCGGAAAAGGAACGAG	ACATGCCGAAGTGATGGAAA	50.0	45.0	58.0	57.8	1214 bp	0.214286	49.6
ycf1_p3	GTTTGCGGAAAAGGAACGAG	AGGATGTCCTTGCCCATACT	50.0	50.0	58.0	58.1	1133 bp	0.071429	41.8
ycf1_p4	TTTGCGGAAAAGGAACGAGA	AGGATGTCCTTGCCCATACT	45.0	50.0	58.0	58.1	1132 bp	0.071429	41.7
ycf1_p5	AGAGGTCCGCAACTTAGACT	AGGATGTCCTTGCCCATACT	50.0	50.0	58.1	58.1	1425 bp	0.071429	41.5