Hotspot

ndhF

SSC · 112563-114788 · 2226 bp

Back to Heptapleurum

Pi 0.00573

MarkerSeek score 40.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 50
pi: 0.00573
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GATGGAATCGCCCATTACGA	CGAACAGGTTGGTCATCTCA	50.0	50.0	57.8	57.5	2785-2820 bp	0.857143	71.9
ndhF_p2	GATGGAATCGCCCATTACGA	ACGAACAGGTTGGTCATCTC	50.0	50.0	57.8	57.5	2786-2821 bp	0.857143	71.9
ndhF_p3	ACATGCCGAAGTGATGGAAA	CGAACAGGTTGGTCATCTCA	45.0	50.0	57.8	57.5	2702-2737 bp	0.857143	71.6
ndhF_p4	AGGATGTCCTTGCCCATACT	CGAACAGGTTGGTCATCTCA	50.0	50.0	58.1	57.5	2621-2656 bp	0.714286	66.1
ndhF_p5	AGGATGTCCTTGCCCATACT	ACGAACAGGTTGGTCATCTC	50.0	50.0	58.1	57.5	2622-2657 bp	0.714286	66.1