Hotspot

rpl32-trnL-TAG

SSC · 116152-116970 · 819 bp

Back to Heptapleurum

Pi 0.00935
MarkerSeek score 52.20
Primer available yes
Label rpl32-trnL-TAG

Feature Metadata

label_name
rpl32-trnL-TAG
feature_type
igs
region
SSC
length_bp
819
variable_sites
32
pi
0.00935
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-TAG_p1 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 939-951 bp 1.0 82.2
rpl32-trnL-TAG_p2 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 940-952 bp 1.0 82.1
rpl32-trnL-TAG_p3 AGGGATATTGGGCAGCCTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 949-961 bp 1.0 81.2
rpl32-trnL-TAG_p4 GCAGCCTTAAAAGCGTTGTC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 928-940 bp 1.0 81.0
rpl32-trnL-TAG_p5 GCAGCCTTAAAAGCGTTGTC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 929-941 bp 1.0 81.0