Hotspot

rpl16-rps3

LSC · 84033-85142 · 1110 bp

Back to Heptapleurum

Pi 0.00647

MarkerSeek score 54.50

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1110
variable_sites: 28
pi: 0.00647
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTTCAAGTGCCTGAAGAGCA	CCTGCAAACCATTCGAGCTA	50.0	50.0	58.4	58.3	1283-1346 bp	1.0	76.7
rpl16-rps3_p2	AGGTTCAAGTGCCTGAAGAG	CCTGCAAACCATTCGAGCTA	50.0	50.0	57.4	58.3	1285-1348 bp	1.0	75.4
rpl16-rps3_p3	TTCAAGTGCCTGAAGAGCAT	CCTGCAAACCATTCGAGCTA	45.0	50.0	57.4	58.3	1282-1345 bp	1.0	75.3
rpl16-rps3_p4	TGTGATCCAAGCAGGTTCAA	CCTGCAAACCATTCGAGCTA	45.0	50.0	57.3	58.3	1297-1360 bp	1.0	74.6
rpl16-rps3_p5	ATCCAAGCAGGTTCAAGTGC	CCTGCAAACCATTCGAGCTA	50.0	50.0	58.7	58.3	1293-1356 bp	1.0	73.9