Hotspot

psaA-ycf3

LSC · 44082-44803 · 722 bp

Back to Heptapleurum

Pi 0.00304

MarkerSeek score 50.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 8
pi: 0.00304
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	886-916 bp	1.0	80.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TATGGCGCTTACTCCTGGTA	50.0	50.0	57.9	57.9	858-888 bp	1.0	80.5
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	888-918 bp	1.0	79.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	815-845 bp	1.0	79.9
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	819-849 bp	1.0	79.7