Hotspot

trnE-TTC-trnT-GGT

LSC · 32872-33693 · 822 bp

Back to Heptapleurum

Pi 0.0116

MarkerSeek score 54.20

Primer available yes

Label trnE-TTC-trnT-GGT

Feature Metadata

label_name: trnE-TTC-trnT-GGT
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 35
pi: 0.0116
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-TTC-trnT-GGT_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	902-934 bp	1.0	84.2
trnE-TTC-trnT-GGT_p2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	939-971 bp	1.0	84.1
trnE-TTC-trnT-GGT_p3	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	867-899 bp	1.0	83.7
trnE-TTC-trnT-GGT_p4	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	904-936 bp	1.0	83.5
trnE-TTC-trnT-GGT_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	880-912 bp	1.0	83.5