Hotspot

rpoB-trnC-GCA

LSC · 28215-29489 · 1275 bp

Back to Heptapleurum

Pi 0.00391

MarkerSeek score 58.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1275
variable_sites: 20
pi: 0.00391
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCTGATCAATGA	CTTACCACTCGGCCATATCG	50.0	55.0	58.0	57.9	1361-1407 bp	1.0	82.6
rpoB-trnC-GCA_p2	CGTCAAGCCCTGATCAATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1401-1447 bp	1.0	82.4
rpoB-trnC-GCA_p3	TCCCTCATTTCCATCTCGGA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1289-1335 bp	1.0	81.6
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCTCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1329-1375 bp	1.0	81.3
rpoB-trnC-GCA_p5	CGTCAAGCCCTGATCAATGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1383-1429 bp	1.0	80.3