Hotspot

ycf1

SSC · 124495-130149 · 5655 bp

Back to Hepatica

Pi 0.00497

MarkerSeek score 44.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5655
variable_sites: 102
pi: 0.00497
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTTTGAATTCTGCGCCTGC	CATATCCGCCCAGTTTGTCA	45.0	50.0	58.0	58.0	1096-1117 bp	1.0	81.9
ycf1_p2	ATTTTGAATTCTGCGCCTGC	ACATATCCGCCCAGTTTGTC	45.0	50.0	58.0	58.0	1097-1118 bp	1.0	81.9
ycf1_p3	TGAAACAGCCCCCAAGTAAC	CATATCCGCCCAGTTTGTCA	50.0	50.0	58.0	58.0	976-991 bp	1.0	81.9
ycf1_p4	TGAAACAGCCCCCAAGTAAC	ACATATCCGCCCAGTTTGTC	50.0	50.0	58.0	58.0	977-992 bp	1.0	81.9
ycf1_p5	GCCCCCAAGTAACCGTTTTA	CATATCCGCCCAGTTTGTCA	50.0	50.0	58.1	58.0	969-984 bp	1.0	81.3