Hotspot

ndhF

SSC · 111367-113562 · 2196 bp

Back to Hepatica

Pi 0.00825

MarkerSeek score 45.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2196
variable_sites: 53
pi: 0.00825
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CATATCCGCCCAGTTTGTCA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2727-2822 bp	1.0	86.0
ndhF_p2	ACATATCCGCCCAGTTTGTC	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2728-2823 bp	1.0	86.0
ndhF_p3	CATATCCGCCCAGTTTGTCA	GAGCTGGTGAATCGGAAACA	50.0	50.0	58.0	58.2	2726-2821 bp	1.0	85.2
ndhF_p4	ACATATCCGCCCAGTTTGTC	GAGCTGGTGAATCGGAAACA	50.0	50.0	58.0	58.2	2727-2822 bp	1.0	85.2
ndhF_p5	CCCCATCGTGGAACAATCAA	CTCACTTAGTTCCCCAACGG	50.0	55.0	58.2	57.9	2896-2981 bp	0.666667	71.2