Hotspot

ndhD-psaC

SSC · 117926-118072 · 147 bp

Back to Hepatica

Pi 0.0354

MarkerSeek score 69.00

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 147
variable_sites: 14
pi: 0.0354
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACCAAGGAAAATCATTCGTGG	GAGACAACTCGTAGCATGGG	42.9	55.0	56.8	58.1	185-260 bp	1.0	70.8
ndhD-psaC_p2	CCAAGGAAAATCATTCGTGGT	GAGACAACTCGTAGCATGGG	42.9	55.0	56.8	58.1	184-259 bp	1.0	70.8
ndhD-psaC_p3	CCAAGGAAAATCATTCGTGGT	TCGTAGCATGGGTCTAGCTT	42.9	50.0	56.8	58.2	176-251 bp	1.0	69.8
ndhD-psaC_p4	ACCAAGGAAAATCATTCGTGG	TCGTAGCATGGGTCTAGCTT	42.9	50.0	56.8	58.2	177-252 bp	1.0	69.7
ndhD-psaC_p5	CCAAGGAAAATCATTCGTGGT	GACAACTCGTAGCATGGGTC	42.9	55.0	56.8	58.4	182-257 bp	1.0	68.5