Hotspot

ndhF-trnL-UAG

SSC · 113563-114968 · 1406 bp

Back to Hepatica

Pi 0.00967

MarkerSeek score 63.90

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1406
variable_sites: 48
pi: 0.00967
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	AAGGGGGACGAAAGGTATGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1469-1768 bp	1.0	85.6
ndhF-trnL-UAG_p2	AAGGGGGACGAAAGGTATGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1470-1769 bp	1.0	85.6
ndhF-trnL-UAG_p3	ACGAAAGGTATGATCCACGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1462-1761 bp	1.0	85.4
ndhF-trnL-UAG_p4	CGAAAGGTATGATCCACGCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1461-1760 bp	1.0	85.4
ndhF-trnL-UAG_p5	ACGAAAGGTATGATCCACGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1463-1762 bp	1.0	85.4