Hotspot

trnL-UAA-trnF-GAA

LSC · 48906-49085 · 180 bp

Back to Hepatica

Pi 0.0153

MarkerSeek score 64.50

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 8
pi: 0.0153
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	263-514 bp	1.0	79.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTAACCACATGCCAGGAACC	50.0	50.0	57.5	58.1	286-537 bp	1.0	79.4
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	CACATGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	281-532 bp	1.0	78.8
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	225-476 bp	1.0	77.1
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	260-511 bp	1.0	76.6