Hotspot

trnG-GCC-trnS-GCU

LSC · 45902-47537 · 1636 bp

Back to Hepatica

Pi 0.00693

MarkerSeek score 61.00

Primer available yes

Label trnG-GCC-trnS-GCU

Feature Metadata

label_name: trnG-GCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 1636
variable_sites: 34
pi: 0.00693
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnS-GCU_p1	AACCGAAAGACCCCTTAACT	TGGACTAAAGCGTCGGATTG	45.0	50.0	56.4	58.0	1782-1955 bp	1.0	72.4
trnG-GCC-trnS-GCU_p2	AACCGAAAGACCCCTTAACT	GTGGACTAAAGCGTCGGATT	45.0	50.0	56.4	58.0	1783-1956 bp	1.0	72.3
trnG-GCC-trnS-GCU_p3	AACCGAAAGACCCCTTAACT	ATTGGGAGAGATGGCTGAGT	45.0	50.0	56.4	58.1	1801-1974 bp	1.0	71.5
trnG-GCC-trnS-GCU_p4	AAACCGAAAGACCCCTTAACT	TGGACTAAAGCGTCGGATTG	42.9	50.0	57.1	58.0	1783-1956 bp	1.0	70.5
trnG-GCC-trnS-GCU_p5	AAACCGAAAGACCCCTTAACT	GTGGACTAAAGCGTCGGATT	42.9	50.0	57.1	58.0	1784-1957 bp	1.0	70.5