Hotspot

atpH-atpF

LSC · 41480-41931 · 452 bp

Back to Hepatica

Pi 0.0175

MarkerSeek score 57.60

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 18
pi: 0.0175
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GTTGTGGCATTAGCGCTTTT	CAGTGACCCAAGGAAACGAA	45.0	50.0	57.9	58.0	511-535 bp	1.0	85.9
atpH-atpF_p2	TGGACTGGTTGTGGCATTAG	CAGTGACCCAAGGAAACGAA	50.0	50.0	57.8	58.0	518-542 bp	1.0	84.8
atpH-atpF_p3	TTTATGGACTGGTTGTGGCA	CAGTGACCCAAGGAAACGAA	45.0	50.0	57.3	58.0	522-546 bp	1.0	81.1
atpH-atpF_p4	GTTGTGGCATTAGCGCTTTT	AGTGACCCAAGGAAACGAAA	45.0	45.0	57.9	57.3	510-534 bp	1.0	80.5
atpH-atpF_p5	TGGACTGGTTGTGGCATTAG	AGTGACCCAAGGAAACGAAA	50.0	45.0	57.8	57.3	517-541 bp	1.0	79.4