Hotspot

trnD-GUC-psbM

LSC · 23768-24889 · 1122 bp

Back to Hepatica

Pi 0.00442

MarkerSeek score 59.30

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1122
variable_sites: 17
pi: 0.00442
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AGTTCAATCGGTCAGAGCAC	TCGTTTTGACTGACCGTTTT	50.0	40.0	57.9	56.1	1219-1315 bp	1.0	69.5
trnD-GUC-psbM_p2	AGTTCAATCGGTCAGAGCAC	AATCGTTTTGACTGACCGTT	50.0	40.0	57.9	55.9	1221-1317 bp	1.0	67.7
trnD-GUC-psbM_p3	AGTTCAATCGGTCAGAGCAC	ATCGTTTTGACTGACCGTTT	50.0	40.0	57.9	55.9	1220-1316 bp	1.0	67.7
trnD-GUC-psbM_p4	AGTTCAATCGGTCAGAGCAC	TCGTTTTGACTGACCGTTTTT	50.0	38.1	57.9	56.9	1219-1315 bp	1.0	67.5
trnD-GUC-psbM_p5	AGTTCAATCGGTCAGAGCAC	AGAATGAACAGTGCAGTAGC	50.0	45.0	57.9	55.8	1165-1261 bp	1.0	66.6