Hotspot

trnT-GGU-trnE-UUC

LSC · 22368-23080 · 713 bp

Back to Hepatica

Pi 0.0125

MarkerSeek score 62.00

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 713
variable_sites: 22
pi: 0.0125
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.0	57.8	854-910 bp	1.0	83.9
trnT-GGU-trnE-UUC_p2	TACCGCTGAGTTAAAAGGGC	TTTCGTAGTACCCTACCCCC	50.0	55.0	57.9	57.8	819-875 bp	1.0	83.3
trnT-GGU-trnE-UUC_p3	GAACCGATGACTTACGCCTT	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.3	816-872 bp	1.0	83.3
trnT-GGU-trnE-UUC_p4	GAACCGATGACTTACGCCTT	TGTCCTGAACCACTAGACGA	50.0	50.0	58.0	57.7	795-851 bp	1.0	83.3
trnT-GGU-trnE-UUC_p5	TGGAAAAAGCCCCTTATCGG	TTTCGTAGTACCCTACCCCC	50.0	55.0	57.9	57.8	878-934 bp	1.0	82.7