Hotspot

psbD-trnT-GGU

LSC · 20935-22295 · 1361 bp

Back to Hepatica

Pi 0.00513
MarkerSeek score 62.70
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
1361
variable_sites
24
pi
0.00513
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 TCTCCGTAACCAGTCATCCA GCCCTTTTAACTCAGCGGTA 50.0 50.0 57.8 57.9 1408-1535 bp 1.0 82.7
psbD-trnT-GGU_p2 TCTCCGTAACCAGTCATCCA GGCGTAAGTCATCGGTTCAA 50.0 50.0 57.8 58.3 1371-1498 bp 1.0 81.3
psbD-trnT-GGU_p3 GGTCTCTCCGTAACCAGTCA GCCCTTTTAACTCAGCGGTA 55.0 50.0 58.5 57.9 1412-1539 bp 1.0 80.8
psbD-trnT-GGU_p4 TCTCCGTAACCAGTCATCCA TAGGCCCTTTTAACTCAGCG 50.0 50.0 57.8 57.6 1411-1538 bp 1.0 80.3
psbD-trnT-GGU_p5 GGTCTCTCCGTAACCAGTCA GGCGTAAGTCATCGGTTCAA 55.0 50.0 58.5 58.3 1375-1502 bp 1.0 79.4