Hotspot

trnG-UCC-psbZ

LSC · 17428-17798 · 371 bp

Back to Hepatica

Pi 0.0154

MarkerSeek score 66.00

Primer available yes

Label trnG-UCC-psbZ

Feature Metadata

label_name: trnG-UCC-psbZ
feature_type: igs
region: LSC
length_bp: 371
variable_sites: 15
pi: 0.0154
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbZ_p1	AATCGAACCCGCATCTTCTC	TTGCTTCTTCTGACGGTTGG	50.0	50.0	58.1	58.4	517-627 bp	1.0	82.4
trnG-UCC-psbZ_p2	GAATCGAACCCGCATCTTCT	TTGCTTCTTCTGACGGTTGG	50.0	50.0	58.1	58.4	518-628 bp	1.0	82.4
trnG-UCC-psbZ_p3	AATCGAACCCGCATCTTCTC	GCTTCTTCTGACGGTTGGTC	50.0	55.0	58.1	58.9	515-625 bp	1.0	78.9
trnG-UCC-psbZ_p4	GAATCGAACCCGCATCTTCT	GCTTCTTCTGACGGTTGGTC	50.0	55.0	58.1	58.9	516-626 bp	1.0	78.9
trnG-UCC-psbZ_p5	GAACCCGCATCTTCTCCTTG	TTGCTTCTTCTGACGGTTGG	55.0	50.0	58.6	58.4	513-623 bp	1.0	77.9