Hotspot

rps16-trnK-UUU

LSC · 1905-2577 · 673 bp

Back to Hepatica

Pi 0.00676
MarkerSeek score 62.60
Primer available yes
Label rps16-trnK-UUU

Feature Metadata

label_name
rps16-trnK-UUU
feature_type
igs
region
LSC
length_bp
673
variable_sites
19
pi
0.00676
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnK-UUU_p1 ATGAAATGCCAAGGACTCGG AAAGCCGAGTACTCTACCGT 50.0 50.0 58.2 58.2 788-866 bp 1.0 82.6
rps16-trnK-UUU_p2 ATGAAATGCCAAGGACTCGG AAGCCGAGTACTCTACCGTT 50.0 50.0 58.2 58.2 787-865 bp 1.0 82.6
rps16-trnK-UUU_p3 ATGAAATGCCAAGGACTCGG TCTACCAACGGTATGGACGA 50.0 50.0 58.2 57.9 853-931 bp 1.0 82.5
rps16-trnK-UUU_p4 CCAAGGACTCGGGATTTTCC TCTACCAACGGTATGGACGA 55.0 50.0 58.2 57.9 845-923 bp 1.0 82.5
rps16-trnK-UUU_p5 CCAAGGACTCGGGATTTTCC AAGCCGAGTACTCTACCGTT 55.0 50.0 58.2 58.2 779-857 bp 1.0 82.5