Hotspot

ycf1

SSC · 126713-132349 · 5637 bp

Back to Hemsleya

Pi 0.00384
MarkerSeek score 35.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5637
variable_sites
41
pi
0.00384
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCGATCTTCATCTTCTGACT TCTAGTGAGAACGTTTGGACA 40.0 42.9 53.2 56.6 246 bp 1.0 42.3
ycf1_p2 TCGATCTTCATCTTCTGACTT TCTAGTGAGAACGTTTGGACA 38.1 42.9 54.1 56.6 246 bp 1.0 42.3
ycf1_p3 TTCGATCTTCATCTTCTGACT TCTAGTGAGAACGTTTGGACA 38.1 42.9 54.1 56.6 247 bp 1.0 42.3
ycf1_p4 TCGATCTTCATCTTCTGACT AGTGAGAACGTTTGGACAAA 40.0 40.0 53.2 55.4 243 bp 1.0 42.3
ycf1_p5 TTCGATCTTCATCTTCTGACTT TCTAGTGAGAACGTTTGGACA 36.4 42.9 55.0 56.6 247 bp 1.0 42.3