Hotspot

rpl16

LSC · 83516-85007 · 1492 bp

Back to Hemsleya

Pi 0.00279

MarkerSeek score 51.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1492
variable_sites: 8
pi: 0.00279
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CTCAAACAAAGACCGCTTTCC	AGACTAAGAAATGACAAATCGGTC	47.6	37.5	58.0	57.0	1625-1627 bp	1.0	40.5
rpl16_p2	CTCAAACAAAGACCGCTTTCC	GACTAAGAAATGACAAATCGGTCT	47.6	37.5	58.0	57.0	1624-1626 bp	1.0	40.5
rpl16_p3	CTCAAACAAAGACCGCTTTCC	ACTAAGAAATGACAAATCGGTCT	47.6	34.8	58.0	55.9	1623-1625 bp	1.0	40.5
rpl16_p4	CTCAAACAAAGACCGCTTTCC	AGACTAAGAAATGACAAATCGGT	47.6	34.8	58.0	55.9	1625-1627 bp	1.0	40.5
rpl16_p5	TCATCTCAAACAAAGACCGCT	GACTAAGAAATGACAAATCGGTCT	42.9	37.5	57.9	57.0	1628-1630 bp	1.0	40.5