Hotspot

trnN-GUU-trnR-ACG

IRa · 132747-133363 · 617 bp

Back to Hemsleya

Pi 0.00513

MarkerSeek score 52.70

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 617
variable_sites: 5
pi: 0.00513
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	759 bp	1.0	78.8
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	796 bp	1.0	78.2
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	710 bp	1.0	77.6
trnN-GUU-trnR-ACG_p4	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	711 bp	1.0	77.2
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	50.0	55.0	58.2	57.7	747 bp	1.0	77.0