Hotspot

ndhF-rpl32

SSC · 115117-115753 · 637 bp

Back to Hemsleya

Pi 0.00654

MarkerSeek score 49.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 637
variable_sites: 8
pi: 0.00654
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	AAAAGCTTTCAACGCTGCTC	45.0	45.0	58.0	57.9	829-832 bp	1.0	79.7
ndhF-rpl32_p2	TGTTGCCGTCGGAAAAAGTA	CGCCCTTTGCTTTTCCAAAT	45.0	45.0	58.0	57.8	807-810 bp	1.0	79.3
ndhF-rpl32_p3	TTTGTTGCCGTCGGAAAAAG	AAAAGCTTTCAACGCTGCTC	45.0	45.0	57.8	57.9	831-834 bp	1.0	78.5
ndhF-rpl32_p4	TTTGTTGCCGTCGGAAAAAG	CGCCCTTTGCTTTTCCAAAT	45.0	45.0	57.8	57.8	809-812 bp	1.0	78.2
ndhF-rpl32_p5	TGTTGCCGTCGGAAAAAGTA	GCGAAAAAGCTTTCAACGCT	45.0	45.0	58.0	58.5	833-836 bp	1.0	76.5